double - stranded dna造句
例句与造句
- the cyanophage contained a double-stranded dna genome at ca . 36.6kb
病毒的遗传物质为双链dna，基因组的大小约36.6kb。 - the genetic material of the new virus is arranged in a similar fashion, encoded in circular, double-stranded dna, and the virus's five proteins have similarities to the proteins of other polyoma viruses
新病毒的遗传物质排列方式与多瘤病毒类似，编码为环状的双链dna，新病毒的五个蛋白质与其他多瘤病毒有相似之处。 - to investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells . methods : we constructed a partial double-strand dna with t7 promoter as dna template and synthesized small hairpinrna in-vitro using t7 rna polymerase
方法：根据端粒酶htert基因1573?1591位的核酸序列，构建带t7启动子的部分双链dna模板，用t7rna聚合酶体外合成短链shrna。 - conclusions : the in-vitro method that partial double-strand dna with t7 wi l .-toi promoter sequence as template and synthesizing by t7 rna polymerase can product high yield, excellent purity shrna . lt is a convenient-, effective ^ low-cost method and fit for small rna synthesis and rna interference researches in ordinary laboratory
结论：以带t7启动子部分双链dna为模板，用t7rna聚合酶体外合成出的shrna产量较高，纯度较好，是一种简便、高效、低成本的短链rna的制备方法，适合于普通实验室用来进行短链rna的合成和rna干扰实验。 - the mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double-stranded dna in genome and form partial double-stranded molecules or triple-stranded nucleic acid molecules by sequence-specific and nonsequence-specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained . in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template . the primers were designed based on 94-500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
本研究设计以ctamrna为模板的反义cdna片段，从cta基因5’端第94位到500位核苷酸段设计引物，目的片段407bp，覆盖第116和188位两个aug密码子，也包含了第一外显子和第二外显子间的剪接位点：用常规分子生物学方法构建了反义片段的腺病毒表达载体(padeasy-1系统)；腺病毒载体经hek293细胞包装产生含反义片段的重组腺病毒，用氯化铯密度梯度离心法获得纯化的高滴度腺病毒；进行体外基因转移，分别用反义片段真核表达载体转染p388d1细胞和用重组腺病毒感染hela细胞，观察导入的cta基因反义rna抑制细胞内组成型或诱导型cta基因表达的作用，从而达到调控mhc-类分子表达的目的。 - It's difficult to find double - stranded dna in a sentence. 用double - stranded dna造句挺难的
- in this study, the suitable parameters for the introduction of plasmids phz1358 and pset152 into s . nanchangensis ns3226 from e . coli were tested for developing an conjugation system for s . nanchangensis ns3226 . a dnd gene cluster, which encodes an unknown modification system for 5 hvidans 1326 and renders its dna susceptible to site-specific double-strand dna cleavage during electrophoresis was conjugated from e . coli into s . nanchangensis ns3226
将克隆在整合型载体pset152上的变铅青链霉菌1326的dnd基因簇通过接合转移导入野生型南昌链霉菌ns3226中进行异源表达，观察到接合转移子的dna获得了在含fe~(2+)的电泳缓冲液中电泳时降解的表型。
